Buchi PrepChrom C-700 Manual de usuario Pagina 78

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6|Operation BÜCHILabortechnikAG
78/110 PrepChromC700
Stepgradientmode
Note
Thefirststepisusedasastartpointonlyandcorrespondstotheconditionused
duringequilibration(ifactivated).Thetimeofthefirststepcannotbemodified.
u Adjusttheflowrate(1).
Note
WhenusingtheBUCHIcolumnmethods,thesystemsetstheoptimalflowrate
forthecolumn(onlyflashcolumns).
u Adjustthepercentageoftheusedsolvents(2).
Note
Alwaysstartadjustingthesolventpercentagesfromtherighttotheleft.
u Addthesecondstep(3).
u Settheendtimeforthesecondstep(4).
Note
Theflowrateandthepercentageoftheusedsolventsinthesecondstepare
equaltothefirststep(=equilibrationstep)andcannotbemodifiedseparately.
u Addotherstepsforelution(3)andadjusttheflowrateandpercentageof
thesolvents.
u SelectthetabDetection.
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